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control scrambled shrna shc002  (Addgene inc)


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    Addgene inc control scrambled shrna shc002
    Control Scrambled Shrna Shc002, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+scrambled+shrna+shc002/pmc03305999-130-46-57?v=Addgene+inc
    Average 85 stars, based on 3 article reviews
    control scrambled shrna shc002 - by Bioz Stars, 2026-07
    85/100 stars

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    scrambled control shrna (shc002)  (Millipore)
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    Millipore scrambled control shrna (shc002)
    STAT3 and LKB1 cooperate to suppress mTORC1. A Western blot analysis of STAT3 and LKB1 in 22Rv1 cells transfected with non-targeting (NT) or shRNAs specific for STAT3 and/or LKB1. B qRT–PCR analysis of STAT3 and STK11 in 22Rv1 cells transfected with NT or <t>shRNA</t> specific for STAT3 ( n = 3 each). C ChIP analysis of STAT3 binding to LKB1 (STK11) promoter. 22Rv1 cells harboring NT or two <t>different</t> <t>shSTAT3</t> constructs were stimulated with IL-6 and immunoprecipated with STAT3 antibody or IgG as a negative control. Bars represent mean ± s.d. from 2 technical replicates. Precipitated DNA is presented as % of input. D IHC analysis of p-4E-BP1, 4E-BP1, p-S6 in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. Scale bars, 100 μm. E Western blots analysis of p-4E-BP1, 4E-BP1, p-S6 and S6 expression in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. β-actin serves as a loading control. F H&E and IHC analyses of Stat3, p-S6 and p-4E-BP1 in prostates and lung metastases from Pten pc + /− and Pten pc + /− Stk11 pc−/− mice, scale bars, 100 μm. Quantification of cells positive for STAT3 and p-4E-BP1 in 19-week-old Pten pc + /− and Pten pc + /− Stk11 pc−/− prostate tissue or lung metastases using HistoQuest software ( n = 3). Data were analyzed by Student’s t -test and are shown as mean ± s.d
    Scrambled Control Shrna (Shc002), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+scrambled+shrna+shc002/pmc10422794-321-27-23?v=Millipore
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    scrambled control shrna (shc002) - by Bioz Stars, 2026-07
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    scrambled shrna plasmid (plko.1-puro non-mammalian shrna control plasmid dna shc002  (Millipore)
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    STAT3 and LKB1 cooperate to suppress mTORC1. A Western blot analysis of STAT3 and LKB1 in 22Rv1 cells transfected with non-targeting (NT) or shRNAs specific for STAT3 and/or LKB1. B qRT–PCR analysis of STAT3 and STK11 in 22Rv1 cells transfected with NT or <t>shRNA</t> specific for STAT3 ( n = 3 each). C ChIP analysis of STAT3 binding to LKB1 (STK11) promoter. 22Rv1 cells harboring NT or two <t>different</t> <t>shSTAT3</t> constructs were stimulated with IL-6 and immunoprecipated with STAT3 antibody or IgG as a negative control. Bars represent mean ± s.d. from 2 technical replicates. Precipitated DNA is presented as % of input. D IHC analysis of p-4E-BP1, 4E-BP1, p-S6 in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. Scale bars, 100 μm. E Western blots analysis of p-4E-BP1, 4E-BP1, p-S6 and S6 expression in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. β-actin serves as a loading control. F H&E and IHC analyses of Stat3, p-S6 and p-4E-BP1 in prostates and lung metastases from Pten pc + /− and Pten pc + /− Stk11 pc−/− mice, scale bars, 100 μm. Quantification of cells positive for STAT3 and p-4E-BP1 in 19-week-old Pten pc + /− and Pten pc + /− Stk11 pc−/− prostate tissue or lung metastases using HistoQuest software ( n = 3). Data were analyzed by Student’s t -test and are shown as mean ± s.d
    Scrambled Shrna Plasmid (Plko.1 Puro Non Mammalian Shrna Control Plasmid Dna Shc002, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    scrambled shrna plasmid (plko.1-puro non-mammalian shrna control plasmid dna shc002 - by Bioz Stars, 2026-07
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    control scrambled shrna (cat. no. shc002)  (Millipore)
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    Millipore control scrambled shrna (cat. no. shc002)
    STAT3 and LKB1 cooperate to suppress mTORC1. A Western blot analysis of STAT3 and LKB1 in 22Rv1 cells transfected with non-targeting (NT) or shRNAs specific for STAT3 and/or LKB1. B qRT–PCR analysis of STAT3 and STK11 in 22Rv1 cells transfected with NT or <t>shRNA</t> specific for STAT3 ( n = 3 each). C ChIP analysis of STAT3 binding to LKB1 (STK11) promoter. 22Rv1 cells harboring NT or two <t>different</t> <t>shSTAT3</t> constructs were stimulated with IL-6 and immunoprecipated with STAT3 antibody or IgG as a negative control. Bars represent mean ± s.d. from 2 technical replicates. Precipitated DNA is presented as % of input. D IHC analysis of p-4E-BP1, 4E-BP1, p-S6 in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. Scale bars, 100 μm. E Western blots analysis of p-4E-BP1, 4E-BP1, p-S6 and S6 expression in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. β-actin serves as a loading control. F H&E and IHC analyses of Stat3, p-S6 and p-4E-BP1 in prostates and lung metastases from Pten pc + /− and Pten pc + /− Stk11 pc−/− mice, scale bars, 100 μm. Quantification of cells positive for STAT3 and p-4E-BP1 in 19-week-old Pten pc + /− and Pten pc + /− Stk11 pc−/− prostate tissue or lung metastases using HistoQuest software ( n = 3). Data were analyzed by Student’s t -test and are shown as mean ± s.d
    Control Scrambled Shrna (Cat. No. Shc002), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+scrambled+shrna+shc002/pmc08577796-200-26-35?v=Millipore
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    control scrambled shrna (cat. no. shc002) - by Bioz Stars, 2026-07
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    plko.1 control shrna (scramble shscr, shc002)  (Millipore)
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    STAT3 and LKB1 cooperate to suppress mTORC1. A Western blot analysis of STAT3 and LKB1 in 22Rv1 cells transfected with non-targeting (NT) or shRNAs specific for STAT3 and/or LKB1. B qRT–PCR analysis of STAT3 and STK11 in 22Rv1 cells transfected with NT or <t>shRNA</t> specific for STAT3 ( n = 3 each). C ChIP analysis of STAT3 binding to LKB1 (STK11) promoter. 22Rv1 cells harboring NT or two <t>different</t> <t>shSTAT3</t> constructs were stimulated with IL-6 and immunoprecipated with STAT3 antibody or IgG as a negative control. Bars represent mean ± s.d. from 2 technical replicates. Precipitated DNA is presented as % of input. D IHC analysis of p-4E-BP1, 4E-BP1, p-S6 in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. Scale bars, 100 μm. E Western blots analysis of p-4E-BP1, 4E-BP1, p-S6 and S6 expression in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. β-actin serves as a loading control. F H&E and IHC analyses of Stat3, p-S6 and p-4E-BP1 in prostates and lung metastases from Pten pc + /− and Pten pc + /− Stk11 pc−/− mice, scale bars, 100 μm. Quantification of cells positive for STAT3 and p-4E-BP1 in 19-week-old Pten pc + /− and Pten pc + /− Stk11 pc−/− prostate tissue or lung metastases using HistoQuest software ( n = 3). Data were analyzed by Student’s t -test and are shown as mean ± s.d
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    plko.1 control shrna (scramble shscr, shc002) - by Bioz Stars, 2026-07
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    control scrambled shrna (shc002)  (Millipore)
    90
    Millipore control scrambled shrna (shc002)
    STAT3 and LKB1 cooperate to suppress mTORC1. A Western blot analysis of STAT3 and LKB1 in 22Rv1 cells transfected with non-targeting (NT) or shRNAs specific for STAT3 and/or LKB1. B qRT–PCR analysis of STAT3 and STK11 in 22Rv1 cells transfected with NT or <t>shRNA</t> specific for STAT3 ( n = 3 each). C ChIP analysis of STAT3 binding to LKB1 (STK11) promoter. 22Rv1 cells harboring NT or two <t>different</t> <t>shSTAT3</t> constructs were stimulated with IL-6 and immunoprecipated with STAT3 antibody or IgG as a negative control. Bars represent mean ± s.d. from 2 technical replicates. Precipitated DNA is presented as % of input. D IHC analysis of p-4E-BP1, 4E-BP1, p-S6 in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. Scale bars, 100 μm. E Western blots analysis of p-4E-BP1, 4E-BP1, p-S6 and S6 expression in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. β-actin serves as a loading control. F H&E and IHC analyses of Stat3, p-S6 and p-4E-BP1 in prostates and lung metastases from Pten pc + /− and Pten pc + /− Stk11 pc−/− mice, scale bars, 100 μm. Quantification of cells positive for STAT3 and p-4E-BP1 in 19-week-old Pten pc + /− and Pten pc + /− Stk11 pc−/− prostate tissue or lung metastases using HistoQuest software ( n = 3). Data were analyzed by Student’s t -test and are shown as mean ± s.d
    Control Scrambled Shrna (Shc002), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+scrambled+shrna+shc002/us09951050-273-30-4?v=Millipore
    Average 90 stars, based on 1 article reviews
    control scrambled shrna (shc002) - by Bioz Stars, 2026-07
    90/100 stars
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    non-target shrna control (scrambled, shc002  (Millipore)
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    Millipore non-target shrna control (scrambled, shc002
    STAT3 and LKB1 cooperate to suppress mTORC1. A Western blot analysis of STAT3 and LKB1 in 22Rv1 cells transfected with non-targeting (NT) or shRNAs specific for STAT3 and/or LKB1. B qRT–PCR analysis of STAT3 and STK11 in 22Rv1 cells transfected with NT or <t>shRNA</t> specific for STAT3 ( n = 3 each). C ChIP analysis of STAT3 binding to LKB1 (STK11) promoter. 22Rv1 cells harboring NT or two <t>different</t> <t>shSTAT3</t> constructs were stimulated with IL-6 and immunoprecipated with STAT3 antibody or IgG as a negative control. Bars represent mean ± s.d. from 2 technical replicates. Precipitated DNA is presented as % of input. D IHC analysis of p-4E-BP1, 4E-BP1, p-S6 in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. Scale bars, 100 μm. E Western blots analysis of p-4E-BP1, 4E-BP1, p-S6 and S6 expression in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. β-actin serves as a loading control. F H&E and IHC analyses of Stat3, p-S6 and p-4E-BP1 in prostates and lung metastases from Pten pc + /− and Pten pc + /− Stk11 pc−/− mice, scale bars, 100 μm. Quantification of cells positive for STAT3 and p-4E-BP1 in 19-week-old Pten pc + /− and Pten pc + /− Stk11 pc−/− prostate tissue or lung metastases using HistoQuest software ( n = 3). Data were analyzed by Student’s t -test and are shown as mean ± s.d
    Non Target Shrna Control (Scrambled, Shc002, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+scrambled+shrna+shc002/pmc03463335-220-0-20?v=Millipore
    Average 90 stars, based on 1 article reviews
    non-target shrna control (scrambled, shc002 - by Bioz Stars, 2026-07
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    control scrambled shrna shc002  (Addgene inc)
    85
    Addgene inc control scrambled shrna shc002
    STAT3 and LKB1 cooperate to suppress mTORC1. A Western blot analysis of STAT3 and LKB1 in 22Rv1 cells transfected with non-targeting (NT) or shRNAs specific for STAT3 and/or LKB1. B qRT–PCR analysis of STAT3 and STK11 in 22Rv1 cells transfected with NT or <t>shRNA</t> specific for STAT3 ( n = 3 each). C ChIP analysis of STAT3 binding to LKB1 (STK11) promoter. 22Rv1 cells harboring NT or two <t>different</t> <t>shSTAT3</t> constructs were stimulated with IL-6 and immunoprecipated with STAT3 antibody or IgG as a negative control. Bars represent mean ± s.d. from 2 technical replicates. Precipitated DNA is presented as % of input. D IHC analysis of p-4E-BP1, 4E-BP1, p-S6 in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. Scale bars, 100 μm. E Western blots analysis of p-4E-BP1, 4E-BP1, p-S6 and S6 expression in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. β-actin serves as a loading control. F H&E and IHC analyses of Stat3, p-S6 and p-4E-BP1 in prostates and lung metastases from Pten pc + /− and Pten pc + /− Stk11 pc−/− mice, scale bars, 100 μm. Quantification of cells positive for STAT3 and p-4E-BP1 in 19-week-old Pten pc + /− and Pten pc + /− Stk11 pc−/− prostate tissue or lung metastases using HistoQuest software ( n = 3). Data were analyzed by Student’s t -test and are shown as mean ± s.d
    Control Scrambled Shrna Shc002, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+scrambled+shrna+shc002/pmc03305999-130-46-57?v=Addgene+inc
    Average 85 stars, based on 1 article reviews
    control scrambled shrna shc002 - by Bioz Stars, 2026-07
    85/100 stars
    		  Buy from Supplier

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    STAT3 and LKB1 cooperate to suppress mTORC1. A Western blot analysis of STAT3 and LKB1 in 22Rv1 cells transfected with non-targeting (NT) or shRNAs specific for STAT3 and/or LKB1. B qRT–PCR analysis of STAT3 and STK11 in 22Rv1 cells transfected with NT or shRNA specific for STAT3 ( n = 3 each). C ChIP analysis of STAT3 binding to LKB1 (STK11) promoter. 22Rv1 cells harboring NT or two different shSTAT3 constructs were stimulated with IL-6 and immunoprecipated with STAT3 antibody or IgG as a negative control. Bars represent mean ± s.d. from 2 technical replicates. Precipitated DNA is presented as % of input. D IHC analysis of p-4E-BP1, 4E-BP1, p-S6 in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. Scale bars, 100 μm. E Western blots analysis of p-4E-BP1, 4E-BP1, p-S6 and S6 expression in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. β-actin serves as a loading control. F H&E and IHC analyses of Stat3, p-S6 and p-4E-BP1 in prostates and lung metastases from Pten pc + /− and Pten pc + /− Stk11 pc−/− mice, scale bars, 100 μm. Quantification of cells positive for STAT3 and p-4E-BP1 in 19-week-old Pten pc + /− and Pten pc + /− Stk11 pc−/− prostate tissue or lung metastases using HistoQuest software ( n = 3). Data were analyzed by Student’s t -test and are shown as mean ± s.d

    Journal: Molecular Cancer

    Article Title: STAT3/LKB1 controls metastatic prostate cancer by regulating mTORC1/CREB pathway

    doi: 10.1186/s12943-023-01825-8

    Figure Lengend Snippet: STAT3 and LKB1 cooperate to suppress mTORC1. A Western blot analysis of STAT3 and LKB1 in 22Rv1 cells transfected with non-targeting (NT) or shRNAs specific for STAT3 and/or LKB1. B qRT–PCR analysis of STAT3 and STK11 in 22Rv1 cells transfected with NT or shRNA specific for STAT3 ( n = 3 each). C ChIP analysis of STAT3 binding to LKB1 (STK11) promoter. 22Rv1 cells harboring NT or two different shSTAT3 constructs were stimulated with IL-6 and immunoprecipated with STAT3 antibody or IgG as a negative control. Bars represent mean ± s.d. from 2 technical replicates. Precipitated DNA is presented as % of input. D IHC analysis of p-4E-BP1, 4E-BP1, p-S6 in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. Scale bars, 100 μm. E Western blots analysis of p-4E-BP1, 4E-BP1, p-S6 and S6 expression in prostates from 19-week-old WT, Pten pc−/− , Pten pc−/− Stat3 pc−/− and Pten pc−/− Stat3 C/ + mice. β-actin serves as a loading control. F H&E and IHC analyses of Stat3, p-S6 and p-4E-BP1 in prostates and lung metastases from Pten pc + /− and Pten pc + /− Stk11 pc−/− mice, scale bars, 100 μm. Quantification of cells positive for STAT3 and p-4E-BP1 in 19-week-old Pten pc + /− and Pten pc + /− Stk11 pc−/− prostate tissue or lung metastases using HistoQuest software ( n = 3). Data were analyzed by Student’s t -test and are shown as mean ± s.d

    Article Snippet: For the knockdown of STAT3 in 22Rv1 and LNCaP cells, the following short hairpin RNA (shRNA) constructs from the Mission TRC shRNA library (Sigma) were used: scrambled control shRNA (SHC002), shSTAT3#456 (TRCN0000071456), shSTAT3#843 (TRCN000002084310.1002/ijc.31724).

    Techniques: Western Blot, Transfection, Quantitative RT-PCR, shRNA, Binding Assay, Construct, Negative Control, Paraffin-embedded Immunohistochemistry, Expressing, Control, Software